Related Experiment Video
Updated: Jul 15, 2025
Mouse Models Of Helicobacter Infection And Gastric Pathologies
Published on: October 18, 2018
Protocol for detecting Helicobacter suis infection in gastric biopsies and serum by PCR and ELISA
Hidenori Matsui1, Masato Suzuki2, Sae Aoki3
1Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo 208-0011, Japan; Department of Bacteriology, Nagoya University Graduate School of Medicine, Aichi 466-8550, Japan; Ōmura Satoshi Memorial Institute, Kitasato University, Tokyo 108-8641, Japan.
Abstract:
Infection with Helicobacter suis, which causes many cases of gastric disease, is not reliably diagnosed. Here, we present a protocol for detecting H. suis infection. We describe steps for collecting gastric biopsies and sera from patients, preparing DNA for PCR, and targeting the H. suis-specific gene. We then define procedures for inoculating biopsies onto primary agar plates and transferring colonies to secondary agar plates. Finally, we detail whole-genome sequencing of bacteria and assess H. suis infection in sera with ELISA. For complete details on the use and execution of these protocols, please refer to Matsui et al.1.
Related Concept Videos
01:28Peptic Ulcer Disease III: Clinical Manifestations and Diagnostic Studies
Few clinical manifestations differentiate gastric ulcers from duodenal ulcers. Distinctions in the location, timing, and pain relief are crucial for healthcare providers in differentiating between gastric and duodenal ulcers during clinical assessments.
01:30Serum Laboratory Studies, Stool Test, Breath Test