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Related Experiment Video

Updated: Jul 15, 2025

Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells
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RBMX involves in telomere stability maintenance by regulating TERRA expression.

Jingfan Liu1, Tian Zheng1,2, Dandan Chen1

  • 1MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.

Plos Genetics
|September 27, 2023
PubMed
Summary
This summary is machine-generated.

The RNA binding protein RBMX regulates telomeric repeat-containing RNA (TERRA) levels. RBMX depletion increases TERRA, causing telomere instability, highlighting RBMX as a potential cancer therapy target.

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Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
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Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Telomeric repeat-containing RNA (TERRA) are lncRNAs transcribed from chromosome ends.
  • TERRA can form R-loops at telomeres, leading to instability if levels are excessive.
  • Mechanisms controlling TERRA levels and telomere integrity are not fully understood.

Purpose of the Study:

  • To identify novel regulators of TERRA levels and telomere integrity.
  • To investigate the role of the RNA binding protein RBMX in TERRA regulation.
  • To explore RBMX as a potential therapeutic target in cancer.

Main Methods:

  • Cellular depletion of RBMX.
  • Analysis of TERRA expression levels.
  • Assessment of telomere R-loop formation and stability.
  • RNA-binding protein immunoprecipitation (RIP) assays.
  • Investigation of TERRA degradation pathways.

Main Results:

  • RBMX depletion significantly elevates TERRA expression.
  • Increased TERRA leads to enhanced telomere R-loop formation, replication stress, and telomere instability.
  • RBMX binds to TERRA and ZCCHC8, promoting TERRA degradation via the nuclear exosome.
  • RBMX depletion slows TERRA degradation.

Conclusions:

  • RBMX is a novel regulator of TERRA expression and telomere integrity.
  • RBMX promotes TERRA degradation, thereby maintaining telomere stability.
  • RBMX represents a potential therapeutic target for cancers associated with telomere dysfunction.