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Existence of lipoprotein lipase in rat brain microvessels
Abstract:
To clarify the possibility that brain microvessels could utilize very low density lipoprotein (VLDL)-triglyceride, the hydrolysis of [14C] triolein in VLDL by rat brain microvessels was examined. The rat brain microvessels were prepared under microscopy. In the homogenate, triolein hydrolyzing activity was observed only in the presence of apolipoprotein C-II. That activity was diminished by the addition of 1 M NaCl. [14C] triolein incorporated in VLDL was hydrolyzed time- dependently. These results suggested that there exists lipoprotein lipase in brain microvessels and the brain can utilize VLDL triglyceride as a source of fatty acids.
Insights
Brain microvessels can use very low-density lipoprotein (VLDL) triglyceride. This suggests the brain utilizes VLDL triglyceride as a fatty acid source via lipoprotein lipase activity.
Area of Science:
- Neuroscience
- Biochemistry
- Metabolism
Background:
- The brain's energy metabolism is complex and not fully understood.
- Investigating the role of lipoproteins in brain energy supply is crucial.
Purpose of the Study:
- To determine if brain microvessels can hydrolyze and utilize very low-density lipoprotein (VLDL)-triglyceride.
- To identify the enzymatic activity responsible for VLDL-triglyceride hydrolysis in brain microvessels.
Main Methods:
- Preparation of rat brain microvessels under microscopy.
- Assay of [14C] triolein hydrolysis in VLDL by microvessel homogenates.
- Investigation of the effect of apolipoprotein C-II and NaCl on hydrolytic activity.
Main Results:
- Triolein hydrolyzing activity was observed in rat brain microvessels.
- Activity required the presence of apolipoprotein C-II.
- Activity was inhibited by high salt concentration (1 M NaCl).
- [14C] triolein within VLDL was hydrolyzed in a time-dependent manner.
Conclusions:
- Brain microvessels possess lipoprotein lipase activity.
- The brain can utilize VLDL-triglyceride as a source of fatty acids.
- This finding provides insight into brain lipid metabolism and energy supply.