[Antibacterial effect of Ankerplast Spray]

Area of Science:

• Microbiology
• Materials Science
• Dermatology

Background:

• Assessing the antimicrobial efficacy of novel spray formulations is crucial for infection control.
• Understanding the activity spectrum of disinfectants against common pathogens is essential.

Purpose of the Study:

• To evaluate the bacteriostatic and bactericidal activity of a novel spray and its solvent.
• To compare the efficacy of the spray against a reference substance (phenol).
• To assess the antimicrobial effect of the spray under a plastic film and in vivo.

Main Methods:

• Tube dilution tests for bacteriostatic and bactericidal activity.
• Agar plate diffusion tests to assess activity under plastic film.
• In vivo experiments on skin to evaluate antibacterial effects.

Main Results:

• The spray and solvent demonstrated bacteriostatic activity against Enterococci, Staphylococcus aureus, Escherichia coli, Proteus, and Pseudomonas aeruginosa.
• Bactericidal activity was observed within 30 minutes, outperforming 1% phenol.
• While some bacteria survived under the film for up to 9 days, potent in vivo antibacterial activity was noted on the skin.

Conclusions:

• The spray exhibits significant antimicrobial properties, surpassing phenol in speed of action.
• The formulation shows promise for topical antimicrobial applications, particularly in vivo.
• Further research may be needed to optimize efficacy against resistant organisms and under occlusive conditions.