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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Jul 15, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Author Correction: Constructing a cost-efficient, high-throughput and high-quality single-molecule localization

John S H Danial^1,2, Jeff Y L Lam^3,4, Yunzhao Wu^3,4

¹Yusuf Hamied Department of Chemistry, University of Cambridge, Cambridge, UK. js2494@cam.ac.uk.

Nature Protocols

|September 27, 2023

Summary

No abstract available in PubMed .

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