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Updated: Jul 15, 2025

Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound
08:51

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Published on: May 8, 2012

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High-Intensity Focused Ultrasound Increases Collagen and Elastin Fiber Synthesis by Modulating Caveolin-1 in Aging

Seyeon Oh1, Do-Young Rhee2, Sosorburam Batsukh1,3

  • 1Functional Cellular Networks Laboratory, Lee Gil Ya Cancer and Diabetes Institute, Gachon University of Medicine, Incheon 21999, Republic of Korea.

Cells
|September 28, 2023
PubMed

Insights

High-intensity focused ultrasound (HIFU) rejuvenates aging skin by reducing caveolin-1 (Cav-1) and p53 activity, boosting collagen and elastin production. This HIFU-induced skin rejuvenation enhances fibroblast proliferation and collagen synthesis.

Area of Science:

  • Dermatology
  • Cell Biology
  • Biomedical Engineering

Background:

  • Cellular senescence, driven by caveolin-1 (Cav-1), impairs skin aging by reducing fibroblast proliferation and collagen synthesis.
  • High-intensity focused ultrasound (HIFU) is known to promote skin rejuvenation and increase collagen production.

Purpose of the Study:

  • To investigate the mechanism by which HIFU enhances collagen production in senescent skin.
  • To determine if HIFU modulates caveolin-1 (Cav-1) levels and related signaling pathways.

Main Methods:

  • Utilized H2O2-induced senescent fibroblasts and aged mouse skin models.
  • Administered HIFU at varying energy levels (0.3, 0.5, 0.7 J) and modes (LINEAR, DOT).
  • Assessed Cav-1 levels, ERK1/2 phosphorylation, p53 activation, cell cycle markers, and collagen/elastin expression.

Main Results:

  • HIFU treatment decreased Cav-1 levels and its binding with MDM2 and Sirt1.
  • HIFU increased ERK1/2 phosphorylation, reduced p53 activation and p21 levels, and elevated cell proliferation markers.
  • Significant increases in collagen and elastin expression and density were observed, with 0.5 J in LINEAR mode being most effective.

Conclusions:

  • HIFU administration rejuvenates aging skin by modulating Cav-1, thereby reducing p53 activity and promoting collagen and elastin synthesis.
  • HIFU's mechanism involves downregulating Cav-1, enhancing ERK1/2 signaling, and inhibiting the senescence pathway.
  • The findings support HIFU as a therapeutic strategy for skin aging and collagenesis.
Keywords:
agingcaveolin-1collagenhigh-intensity focused ultrasoundp53 activityskin rejuvenation

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