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CAM Model: Intriguing Natural Bioreactor for Sustainable Research and Reliable/Versatile Testing.

Carla Palumbo¹, Federica Sisi¹, Marta Checchi¹

¹Department of Biomedical, Metabolic and Neural Sciences, Section of Human Morphology, University of Modena and Reggio Emilia-Largo del Pozzo, 41124 Modena, Italy.

|September 28, 2023

Summary

The chick embryo chorioallantoic membrane (CAM) assay offers a refined, sustainable model bridging in vitro and in vivo research for drug development and tissue engineering. Its applications include testing drug efficacy and validating biomaterials for regenerative medicine.

Keywords:

angiogenesis chorioallantoic membrane (CAM)engineered 3D scaffold organotypic culture

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Area of Science:

Biomedical Engineering
Developmental Biology
Pharmacology

Background:

The chorioallantoic membrane (CAM) assay is experiencing a revival as a versatile model.
It bridges the gap between in vitro and in vivo studies for translational research.
The CAM assay supports the
reduce
, and
refine
(3Rs) principles in pre-clinical research.

Purpose of the Study:

To highlight the unique potential of the CAM assay in various research areas.
To propose the future use of the CAM model for evaluating the angiogenic capacity of 3D constructs in regenerative medicine.
To assess the efficacy of these constructs for critical-size skeletal defect (CSD) repair.

Main Methods:

The CAM assay allows for testing cytotoxicity, pharmacokinetics, tumorigenesis, invasion, and molecular mechanisms.
It is used for validating new materials in tissue engineering.
Future applications involve organotypic cultures on CAMs and a CAM bioreactor for CSD assessment.

Main Results:

The CAM assay provides a simplified, in vivo-like microenvironment for experimentation.
It offers fast outcomes and ease of use.
The model enhances experimental sustainability by reducing and refining animal testing.

Conclusions:

The CAM assay is a valuable and sustainable tool for pre-clinical research and drug development.
Its application in regenerative medicine for CSD repair shows significant promise.
The CAM model facilitates the validation of novel biomaterials and therapeutic strategies in a biologically relevant context.