CAM Model: Intriguing Natural Bioreactor for Sustainable Research and Reliable/Versatile Testing

Area of Science:

• Biomedical Engineering
• Developmental Biology
• Pharmacology

Background:

• The chorioallantoic membrane (CAM) assay is experiencing a revival as a versatile model.
• It bridges the gap between in vitro and in vivo studies for translational research.
• The CAM assay supports the
• reduce
• , and
• refine
• (3Rs) principles in pre-clinical research.

Purpose of the Study:

• To highlight the unique potential of the CAM assay in various research areas.
• To propose the future use of the CAM model for evaluating the angiogenic capacity of 3D constructs in regenerative medicine.
• To assess the efficacy of these constructs for critical-size skeletal defect (CSD) repair.

Main Methods:

• The CAM assay allows for testing cytotoxicity, pharmacokinetics, tumorigenesis, invasion, and molecular mechanisms.
• It is used for validating new materials in tissue engineering.
• Future applications involve organotypic cultures on CAMs and a CAM bioreactor for CSD assessment.

Main Results:

• The CAM assay provides a simplified, in vivo-like microenvironment for experimentation.
• It offers fast outcomes and ease of use.
• The model enhances experimental sustainability by reducing and refining animal testing.

Conclusions:

• The CAM assay is a valuable and sustainable tool for pre-clinical research and drug development.
• Its application in regenerative medicine for CSD repair shows significant promise.
• The CAM model facilitates the validation of novel biomaterials and therapeutic strategies in a biologically relevant context.