Related Experiment Video
Updated: Jul 15, 2025
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Exploring Longitudinal Gut Microbiome towards Metabolic Functional Changes Associated in Atopic Dermatitis in Early
Preecha Patumcharoenpol1, Amornthep Kingkaw1, Massalin Nakphaichit2
1Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
Insights
Early childhood atopic dermatitis (AD) is linked to specific gut bacteria, even without changes in overall gut microbiome diversity. This study highlights key bacterial differences and metabolic functions in Thai children with AD.
Area of Science:
- Microbiology
- Immunology
- Pediatric Health
Background:
- Atopic dermatitis (AD) is a common inflammatory skin condition.
- Gut microbiome alterations in early life are linked to AD.
- Limited longitudinal studies exist on the gut microbiome in AD.
Purpose of the Study:
- To investigate taxonomic and metabolic functions of longitudinal gut microbiomes in Thai children with AD.
- To analyze gut microbial changes from 9 to 30 months of age.
- To establish a framework for monitoring microbial imbalances in allergic diseases.
Main Methods:
- Longitudinal analysis of gut microbiomes in Thai children (9-30 months).
- Integrative data analysis to compare healthy and AD groups.
- Taxonomic and functional profiling of microbial communities.
Main Results:
- No significant difference in gut microbiome diversity between healthy and AD groups.
- Increased abundance of SCFA-producing bacteria (e.g., *Anaerostipes*, *Butyricicoccus*, *Ruminococcus*, *Lactobacillus*) in the AD group.
- Observed metabolic alterations related to vitamin production and immune response (e.g., menaquinol, succinate, (Kdo)2-lipid A biosynthesis).
Conclusions:
- Specific gut bacteria and metabolic functions are associated with AD in Thai children.
- Longitudinal microbiome analysis provides insights into AD development.
- This study offers a framework for understanding microbial roles in pediatric allergic diseases.
Abstract:
Atopic dermatitis (AD) is a prevalent inflammatory skin disease that has been associated with changes in gut microbial composition in early life. However, there are limited longitudinal studies examining the gut microbiome in AD. This study aimed to explore taxonomy and metabolic functions across longitudinal gut microbiomes associated with AD in early childhood from 9 to 30 months of age using integrative data analysis within the Thai population. Our analysis revealed that gut microbiome diversity was not different between healthy and AD groups; however, significant taxonomic differences were observed. Key gut bacteria with short-chain fatty acids (SCFAs) production potentials, such as Anaerostipes, Butyricicoccus, Ruminococcus, and Lactobacillus species, showed a higher abundance in the AD group. In addition, metabolic alterations between the healthy and AD groups associated with vitamin production and host immune response, such as biosynthesis of menaquinol, succinate, and (Kdo)2-lipid A, were observed. This study serves as the first framework for monitoring longitudinal microbial imbalances and metabolic functions associated with allergic diseases in Thai children during early childhood.
More Related Videos
09:32Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
Published on: September 26, 2019
05:31Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels
Published on: August 7, 2017
