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Updated: Jul 15, 2025

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Euphorbiasteroid Induces Apoptosis as Well as Autophagy through Modulating SHP-1/STAT3 Pathway in Hepatocellular

Na Young Kim1, Gautam Sethi2, Jae-Young Um1

  • 1Department of Science in Korean Medicine, Kyung Hee University, 24 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.

International Journal of Molecular Sciences
|September 28, 2023
PubMed

Insights

Euphorbiasteroid (EPBS) shows potential as an anti-cancer agent for hepatocellular carcinoma (HCC). It induces apoptosis and autophagy by targeting the SHP-1/STAT3 pathway, offering a new therapeutic strategy for HCC.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Hepatocellular carcinoma (HCC) remains a significant global health challenge.
  • The anti-cancer properties of Euphorbiasteroid (EPBS) have been noted in lung cancer and sarcoma, but its effect on HCC is unknown.

Purpose of the Study:

  • To investigate the cytotoxic effects of Euphorbiasteroid (EPBS) on human hepatocellular carcinoma (HCC) cells.
  • To elucidate the molecular mechanisms underlying EPBS's action in HCC.

Main Methods:

  • Treatment of HCC cells with EPBS.
  • Analysis of apoptosis and autophagy induction.
  • Assessment of signal transducer and activator of transcription 3 (STAT3) protein expression.
  • Evaluation of SHP-1 protein expression and reactive oxidative stress (ROS) production.
  • Silencing of SHP-1 using siRNA to observe the reversal of EPBS effects.

Main Results:

  • EPBS induced apoptosis and autophagy in HCC cells.
  • EPBS suppressed both constitutive and inducible STAT3 activation.
  • EPBS treatment increased SHP-1 protein expression and ROS production.
  • Knockdown of SHP-1 reversed the apoptosis- and autophagy-inducing effects of EPBS.

Conclusions:

  • EPBS exhibits anti-cancer potential against HCC by inducing apoptosis and autophagy.
  • The SHP-1/STAT3 signaling pathway is a key target for EPBS's anti-cancer activity in HCC.
  • EPBS represents a promising therapeutic candidate for hepatocellular carcinoma treatment.
Keywords:
SHP-1STAT3apoptosisautophagyeuphorbiasteroid

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