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A Multiplexed Urinary Biomarker Panel Has Potential for Alzheimer's Disease Diagnosis Using Targeted Proteomics and
Jenny Hällqvist1, Rui C Pinto2, Wendy E Heywood1
1Translational Mass Spectrometry Research Group, Genetics and Genomic Medicine, UCL Great Ormond Street Institute of Child Health, London WC1N 1EH, UK.
International Journal of Molecular Sciences
|September 28, 2023
Summary
A new urine test using mass spectrometry shows promise for diagnosing Alzheimer's disease (AD). This accessible screening tool identified key protein biomarkers with 86% accuracy, aiding early AD detection.
Area of Science:
- Biomarker discovery
- Proteomics
- Neurodegenerative disease diagnostics
Background:
- Accessible, accurate, and affordable Alzheimer's disease (AD) biomarkers are crucial with the advent of disease-modifying therapies.
- Current diagnostic methods can be invasive or costly, highlighting the need for high-throughput screening tools.
Purpose of the Study:
- To develop a mass spectrometry-based urine test for high-throughput screening and diagnosis of Alzheimer's disease.
- To discover and validate protein biomarkers in urine for AD detection.
Main Methods:
- Untargeted proteomics was employed for biomarker discovery in a cohort of individuals with AD and their study partners.
- A targeted, multiplexed proteomic assay was developed using identified biomarkers and tested on an independent cohort.
Main Results:
- A panel of proteins, including MIEN1, TNFB, VCAM1, REG1B, and ABCA7, was identified.
- This protein panel achieved an 86% classification accuracy in distinguishing AD patients from controls.
- The identified proteins are known to be involved in Alzheimer's disease pathogenesis.
Conclusions:
- Urine-targeted mass spectrometry demonstrates significant potential as a diagnostic screening tool for Alzheimer's disease.
- This approach offers a non-invasive and potentially cost-effective method for AD detection.
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