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Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
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miR-383-5p Regulates Preadipocyte Proliferation and Differentiation by Targeting RAD51AP1

Meigui Wang1, Jiahao Shao1, Xiaoxiao Zhang1

  • 1College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

International Journal of Molecular Sciences
|September 28, 2023
PubMed

Insights

MicroRNAs (miRNAs) like miR-383-5p play a role in obesity by influencing lipid metabolism. This study shows miR-383-5p promotes preadipocyte differentiation and lipid accumulation by targeting RAD51AP1.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Obesity is a global health issue with microRNAs (miRNAs) implicated in its development.
  • miR-383-5p is upregulated in perirenal fat of rabbits fed high-fat diets, suggesting a role in lipid metabolism.

Purpose of the Study:

  • To investigate the role of miR-383-5p in lipid metabolism and adipogenesis.
  • To identify the molecular targets and mechanisms through which miR-383-5p regulates lipid accumulation.

Main Methods:

  • Transcriptome sequencing to identify differentially expressed genes between miR-383-5p mimic and control groups.
  • Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis.
  • Experimental validation of miR-383-5p's effect on rabbit preadipocyte proliferation, differentiation, and its targeting relationship with RAD51AP1.

Main Results:

  • Transcriptome analysis revealed enrichment of lipid metabolism, glycine biosynthesis, NOD receptor signaling, and nonalcoholic fatty liver pathways.
  • miR-383-5p was found to promote the proliferation and differentiation of rabbit preadipocytes.
  • A direct targeting relationship between miR-383-5p and RAD51AP1 was identified, linking miR-383-5p to lipid metabolism regulation.

Conclusions:

  • miR-383-5p plays a significant role in regulating lipid metabolism and adipogenesis.
  • The miR-383-5p/RAD51AP1 axis is a potential mechanism contributing to lipid accumulation in obesity.
  • Targeting the miR-383-5p/RAD51AP1 pathway could offer novel strategies for obesity management.
Keywords:
RAD51AP1differentiationmiR-383-5ppreadipocytesproliferationrabbits

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