Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Benzo[b]fluoranthene Induces Mutation Accumulation and Cancer-Relevant Mutational Signatures in Mouse Lung Alongside Steady State Levels of Chromosome Damage in Blood.

Environmental and molecular mutagenesis·2026
Same author

Immune receptor LAG3 regulates microglia function during Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same author

Alzheimer's Disease Risk Allele <i>APOE4</i> Interacts with Arsenic Exposure to Drive Microglial Dysfunction.

bioRxiv : the preprint server for biology·2026
Same author

Placental Lesions in CD-1 Mouse Placenta Following Gestational Exposure to Perfluorooctanoic Acid (PFOA) or Hexafluoropropylene Oxide Dimer Acid (HFPO-DA or GenX).

Toxicologic pathology·2026
Same author

Immune receptor LAG3 regulates microglia function during Alzheimer's disease.

Neurobiology of disease·2026
Same author

Correction: Genetic profiling of rat gliomas and cardiac schwannomas from life-time radiofrequency radiation exposure study using a targeted next-generation sequencing gene panel.

PloS one·2026

Related Experiment Video

Updated: Jun 30, 2026

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
18:30

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Published on: February 13, 2013

22.0K

Insights into Repeated Renal Injury Using RNA-Seq with Two New RPTEC Cell Lines.

B Alex Merrick1, Negin P Martin2, Ashley M Brooks3

  • 1Mechanistic Toxicology Branch, Division of Translational Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA.

International Journal of Molecular Sciences
|September 28, 2023
PubMed
Summary
This summary is machine-generated.

Two immortalized renal proximal tubule epithelial cell lines were developed to model kidney injury. Cisplatin induced a coordinated injury and repair response, while aflatoxin B1 showed specific nephrotoxicity in one cell line.

Keywords:
RNA-seqaflatoxin B1cisplatinimmortalizationkidneynephrogenesisnephrotoxicityrenal proximal tubuletranscriptomics

More Related Videos

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers
12:22

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers

Published on: January 22, 2013

33.6K
Microfluidic Co-culture of Renal Healthy and Tumor Epithelium to Model Kidney Cancer Progression
06:29

Microfluidic Co-culture of Renal Healthy and Tumor Epithelium to Model Kidney Cancer Progression

Published on: January 31, 2025

646

Related Experiment Videos

Last Updated: Jun 30, 2026

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
18:30

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Published on: February 13, 2013

22.0K
The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers
12:22

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers

Published on: January 22, 2013

33.6K
Microfluidic Co-culture of Renal Healthy and Tumor Epithelium to Model Kidney Cancer Progression
06:29

Microfluidic Co-culture of Renal Healthy and Tumor Epithelium to Model Kidney Cancer Progression

Published on: January 31, 2025

646

Area of Science:

  • Nephrology
  • Toxicology
  • Cell Biology

Background:

  • Renal proximal tubule epithelial cells (RPTECs) are crucial in kidney injury.
  • Understanding cellular responses to toxicants is vital for nephroprotection.
  • Immortalized cell lines offer valuable in vitro models for studying kidney disease.

Purpose of the Study:

  • To establish and characterize two distinct RPTEC cell lines immortalized with hTERT or SV40 LgT.
  • To investigate the differential gene expression and pathway activation in response to sub-lethal cisplatin (CisPt) and aflatoxin B1 (AFB1) exposure.
  • To assess the utility of these cell lines as in vitro models for studying mechanisms of kidney injury and repair.

Main Methods:

  • Creation of two RPTEC lines from CD-1 mice, immortalized using hTERT or SV40 LgT.
  • Exposure of cell lines to low-level, repeated doses of CisPt or AFB1.
  • RNA sequencing (RNA-seq) to analyze differential gene expression (DEGs) and pathway analysis.

Main Results:

  • SV40 LgT immortalized cells uniquely responded to AFB1, indicating direct nephrotoxic properties.
  • Both cell lines exhibited robust transcriptional responses to CisPt, involving immune signaling, cell adhesion, and nephrogenesis pathways.
  • Cisplatin exposure triggered a coordinated transcriptional program indicative of injury signaling and repair mechanisms.

Conclusions:

  • The developed RPTEC cell lines serve as effective in vitro models for studying repeated kidney injury and repair.
  • Distinct cellular responses to CisPt and AFB1 highlight the importance of cell line choice in toxicological studies.
  • These models facilitate a deeper understanding of nephrotoxicity and potential therapeutic targets.