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Updated: Jul 15, 2025
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
34.0K
Evolution of CRISPR/Cas Systems for Precise Genome Editing
Magdalena Hryhorowicz1, Daniel Lipiński1, Joanna Zeyland1
1Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznań, Poland.
International Journal of Molecular Sciences
|September 28, 2023
Summary
The CRISPR/Cas system offers versatile genome engineering for various applications. This review details its classification, precise editing technologies, applications, and remaining challenges for future development.
Area of Science:
- Molecular Biology
- Biotechnology
- Genetics
Background:
- The CRISPR/Cas system, derived from bacteria, is a leading tool for genome engineering.
- Its applications span disease modeling, gene therapy, transcriptional modulation, and diagnostics.
- Current limitations include potential immunological reactions and off-target effects.
Purpose of the Study:
- To classify CRISPR systems and outline precise genome-editing technologies.
- To summarize recent applications of CRISPR/Cas in diverse research fields.
- To discuss limitations, ethical considerations, and future challenges of CRISPR/Cas technology.
Main Methods:
- Literature review of CRISPR/Cas systems and their applications.
- Analysis of current genome-editing technologies and their precision.
- Discussion of challenges, ethical issues, and future directions.
Main Results:
- Detailed classification of various CRISPR systems.
- Overview of advanced, precise genome-editing tools.
- Summary of CRISPR/Cas applications in medicine and research.
- Identification of key limitations and ethical considerations.
Conclusions:
- CRISPR/Cas technology is a powerful and evolving tool for genome engineering.
- Ongoing research focuses on enhancing precision and mitigating risks.
- Addressing limitations and ethical concerns is crucial for responsible advancement.
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