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Unveiling the Significance of FGF8 Overexpression in Orchestrating the Progression of Ovarian Cancer

Kumari Binita Chandra1, Vikrant Kumar1, Swati Ranjan1

  • 1Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India.

International Journal of Molecular Sciences
|September 28, 2023
PubMed

Insights

Fibroblast growth factor 8 (FGF8) is overexpressed in epithelial ovarian cancer (EOC) and promotes metastasis. Silencing FGF8 reduced ovarian cancer cell survival, migration, and invasion, highlighting its role in EOC development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Ovarian cancer recurrence and chemoresistance necessitate novel therapeutic targets.
  • Fibroblast growth factor 8 (FGF8) is implicated in ovarian cancer development and metastasis, but its precise role requires elucidation.

Purpose of the Study:

  • To investigate the expression levels of FGF8 in epithelial ovarian cancer (EOC) patients.
  • To determine the functional role of FGF8 in ovarian cancer cell survival, adhesion, migration, and invasion.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) were used to assess FGF8 expression in patient saliva and tissue samples.
  • In vitro cell assays were performed on SKOV3 cells following FGF8 gene silencing to evaluate effects on cell survival, adhesion, migration, and invasion.

Main Results:

  • A stepwise increase in FGF8 expression was observed in saliva samples correlating with disease progression from controls to high-grade EOC.
  • Elevated FGF8 protein and mRNA levels were detected in EOC tissues compared to controls.
  • FGF8 gene silencing significantly impaired SKOV3 cell survival, extracellular matrix adhesion, migration, and invasion.

Conclusions:

  • FGF8 exhibits a pro-metastatic function in epithelial ovarian cancer.
  • FGF8 is a potential therapeutic target for managing ovarian cancer progression and metastasis.
Keywords:
FGF8cell invasioncell migrationgene silencingovarian cancer

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