Reinforcement of Different Sands by Low-pH Bio-Mineralization

Different sands have significant influences on MICP reinforcement effects. Using calcium carbonate production and bioflocculation lag period as evaluation criteria, this study investigates the optimal theoretical pH values of bacterial solutions with different concentrations. We reinforced four different sands using MICP at the optimal theoretical pH, and based on permeability, moisture retention, raindrop erosion, wind erosion, penetration, and SEM tests, the influence of sand properties on low-pH MICP reinforcement was analyzed and the low-pH MICP mechanism was revealed. The results indicate the following: (1) The optimal theoretical pH values for bacterial solutions with concentrations of 0.67 × 10⁸ cells/mL, 3 × 10⁸ cells/mL, and 10 × 10⁸ cells/mL are 4.5, 3, and 4, respectively. (2) With 0.67 × 10⁸, 3 × 10⁸, and 10 × 10⁸ cells/mL bacterial solutions, the strength of tailings sand containing calcium salt was 21.15%, 44.42%, and 13.61% higher than that of quartz sand, respectively. The effective reinforcement depth of alkaline reclaimed sand was 10, 8, and 6 mm lower than that of neutral calcareous sand, respectively. The strength of fine tailings sand was 70.41%, 58.04%, and 22.6% higher than that of coarse reclaimed sand. The effective reinforcement depth of fine quartz sand was 6, 4, and 4 mm lower than that of coarse calcareous sand. (3) Low pH temporarily suppresses urease activity, delaying calcium carbonate flocculation and enhancing reinforcement uniformity. To achieve optimal reinforcement effects, adjusting the actual optimal pH values of bacterial solution based on sand properties is essential in engineering applications.