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Paper-Based Aptasensors: Working Principles, Detection Modes, and Applications.

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This review explores paper-based aptasensors, which combine aptamers and paper devices for low-cost, on-site detection. These aptasensors offer a promising alternative to traditional methods for various analytes.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Materials Science

Background:

  • Aptamers are highly specific oligonucleotide-based bioreceptors, offering advantages over antibodies in bioassays and biosensors.
  • Paper-based analytical devices (PADs) provide a low-cost, portable platform for on-site and point-of-care diagnostics.
  • Combining aptamers with PADs creates paper-based aptasensors, enhancing diagnostic capabilities.

Purpose of the Study:

  • To provide a comprehensive overview of paper-based aptasensors.
  • To discuss the fabrication, working principles, and detection modes of these devices.
  • To highlight applications and future prospects in analyte detection.

Main Methods:

  • Review of literature on paper-based aptasensor fabrication and design.
  • Analysis of aptamer properties as biorecognition elements.
  • Discussion of various detection strategies employed in aptasensing PADs.

Main Results:

  • Paper-based aptasensors integrate aptamers with PADs for sensitive and specific detection.
  • Diverse applications demonstrated for detecting ions, small molecules, proteins, and cells.
  • The review covers fabrication methods, working principles, and detection modes.

Conclusions:

  • Paper-based aptasensors represent a significant advancement in low-cost, portable diagnostic tools.
  • These devices offer a viable alternative to traditional bioassays for point-of-care applications.
  • Future research should address challenges and explore new prospects for enhanced performance and broader utility.