Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Refractory <i>Candidozyma</i> (<i>Candida</i>) <i>auris</i>-associated central nervous system infection in a postoperative neurosurgical patient.

ASM case reports·2026
Same author

High-throughput multiplex detection of respiratory pathogens: multicenter evaluation of the NxTAG Respiratory Pathogen Panel v2 assay.

Journal of clinical microbiology·2026
Same author

Diagnostic Approaches for Measles Virus: Methods, Advances, and Ongoing Challenges.

Pathogens (Basel, Switzerland)·2025
Same author

Author Correction: Nasal delivery of an IgM offers broad protection from SARS-CoV-2 variants.

Nature·2025
Same author

Evaluation of a Novel Rapid Phenotypic Antimicrobial Susceptibility Testing System.

Antibiotics (Basel, Switzerland)·2025
Same author

Photo Quiz: Unexpected yeast in a premature infant-pathogen or passenger.

Journal of clinical microbiology·2025

Related Experiment Video

Updated: Jul 15, 2025

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
07:08

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

5.1K

An Integrated Research-Clinical BSL-2 Platform for a Live SARS-CoV-2 Neutralization Assay.

Jing Zou1, Chaitanya Kurhade2, Hope C Chang2

  • 1Department of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555, USA.

Viruses
|September 28, 2023
PubMed
Summary
This summary is machine-generated.

We developed a safe and rapid live SARS-CoV-2 neutralization assay using an attenuated virus. This platform efficiently detects neutralizing antibodies against SARS-CoV-2 variants of concern (VOCs) within 24 hours.

Keywords:
COVID-19SARS-CoV-2high throughputlive attenuatedneutralization assayserological diagnosisvariants of concern

More Related Videos

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

4.7K
Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research
06:08

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Published on: September 8, 2023

1.2K

Related Experiment Videos

Last Updated: Jul 15, 2025

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
07:08

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

5.1K
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

4.7K
Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research
06:08

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Published on: September 8, 2023

1.2K

Area of Science:

  • Virology
  • Immunology
  • Public Health

Background:

  • Monitoring neutralizing antibodies against SARS-CoV-2 and its variants of concern (VOCs) requires reliable and efficient serological tests.
  • Existing assays may have limitations in terms of safety, speed, or scope for variant detection.

Purpose of the Study:

  • To present an integrated research-clinical platform for a live SARS-CoV-2 neutralization assay.
  • To enable safe handling and rapid assessment of neutralization titers against SARS-CoV-2 and VOCs.

Main Methods:

  • Development of a highly attenuated SARS-CoV-2 strain (Δ3678_WA1-spike) for safe use in biosafety level 2 (BSL-2) laboratories.
  • Construction of genetically stable reporter viruses (mGFP Δ3678_WA1-spike, mGFP Δ3678_BA.5-spike, mGFP Δ3678_XBB.1.5-spike) incorporating a modified green fluorescent protein (mGFP).
  • Optimization of a fluorescence reduction neutralization assay and a robot-aided workflow for rapid (24h) and accurate assessment of neutralization titers.

Main Results:

  • The reporter viruses exhibited robust fluorescent signals and neutralization titers that correlated highly with conventional plaque reduction assays.
  • The robot-aided workflow demonstrated sensitivity, specificity, reproducibility, and accuracy.
  • The assay successfully assessed neutralization titers against SARS-CoV-2 variants within 24 hours of sample receipt.

Conclusions:

  • The developed platform offers a valuable tool for large-scale testing of clinical samples against SARS-CoV-2 and VOCs at BSL-2.
  • This innovative approach supports pandemic preparedness and response strategies by enabling rapid monitoring of antibody responses.
  • The assay's safety, efficiency, and accuracy make it suitable for clinical and research applications.