Purification and functional validation of LtCas12a protein
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Updated: Jul 15, 2025
Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Published on: December 12, 2017
Bo Zhou1, Ye Chen2, Lifang Li2
1Department of Gynecological Oncology, Zhongnan Hospital of Wuhan University, Wuhan 430062, Hubei, China; Women and Children's Hospital Affiliated to Zhongnan Hospital of Wuhan University, Wuhan 430062, Hubei, China.
Abstract:
Here, we present a protocol for generating LtCas12a protein recognizing distinct TTNA (N represented A, T, C, G) protospacer adjacent motif sequence. We describe steps for transforming and harvesting bacterial cells and protein purification including nickel affinity chromatography and dialysis. We then detail procedures for verification of LtCas12a with cis- and trans-cleavage activities. For complete details on the use and execution of this protocol, please refer to Chen et al. (2023).1.
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