Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

42
Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
42
Protein Translocation Machinery on the ER Membrane01:28

Protein Translocation Machinery on the ER Membrane

4.7K
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
4.7K
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

36
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
36
Protein Complex Assembly02:41

Protein Complex Assembly

10.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.6K
Mechanism of Conjugation01:19

Mechanism of Conjugation

36
Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...
36
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

7.1K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
7.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Bacillales-specific tubular scaffold essential for NADH dehydrogenase activity.

Nature communications·2026
Same author

JUN Specifies the Local Differentiation and Maintenance of Pro-tumoral Monocyte-Derived Macrophages.

Cancer research·2026
Same author

The NMR Exchange Format (NEF): Specification and Applications.

bioRxiv : the preprint server for biology·2026
Same author

Structural determinants of endopilus assembly, stability and functional specificity in bacterial type II secretion.

bioRxiv : the preprint server for biology·2026
Same author

Interpreting chemical crosslinks: Score-based approaches and deep neural networks.

Current opinion in structural biology·2026
Same author

Demonstration of the Role of Both a Ttr and a Psr Homologue Enzymes in the Respiration of Tetrathionate by an Environmental Bacterium Shewanella sp. ANA-3.

Environmental microbiology·2026

Related Experiment Video

Updated: Jul 15, 2025

A Visual Assay to Monitor T6SS-mediated Bacterial Competition
08:45

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

Published on: March 20, 2013

15.7K

Acinetobacter type VI secretion system comprises a non-canonical membrane complex.

Ona Kandolo1, Yassine Cherrak1, Isaac Filella-Merce2,3

  • 1Laboratoire d'Ingénierie des Systèmes Macromoléculaires (LISM), Institut de Microbiologie, Bioénergies and Biotechnologie (IM2B), Aix-Marseille Université, Centre National de la Recherche Scientifique (CNRS)-UMR 7255, Marseille, France.

Plos Pathogens
|September 28, 2023
PubMed
Summary
This summary is machine-generated.

Acinetobacter baumannii

More Related Videos

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
10:41

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Published on: January 4, 2017

13.9K
Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
11:33

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking

Published on: December 17, 2013

6.2K

Related Experiment Videos

Last Updated: Jul 15, 2025

A Visual Assay to Monitor T6SS-mediated Bacterial Competition
08:45

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

Published on: March 20, 2013

15.7K
Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
10:41

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Published on: January 4, 2017

13.9K
Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
11:33

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking

Published on: December 17, 2013

6.2K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Pathogenesis

Background:

  • Acinetobacter baumannii is a significant opportunistic pathogen known for rapid acquisition of resistance.
  • The type VI secretion system (T6SS) in Acinetobacter plays roles in motility and interbacterial competition.
  • While T6SS regulation is studied, its assembly and architecture, particularly the membrane complex (MC), remain poorly understood.

Purpose of the Study:

  • To characterize the A. baumannii T6SS membrane complex (MC) and understand how it assembles without the TssJ lipoprotein.
  • To identify the specific proteins involved in the A. baumannii MC biogenesis.
  • To elucidate the mechanisms A. baumannii employs to compensate for the absence of TssJ.

Main Methods:

  • Proteomic analysis to define MC composition.
  • Biochemical assays to study protein interactions and localization.
  • Microscopy techniques to visualize MC assembly dynamics.

Main Results:

  • The A. baumannii MC is composed of five proteins, utilizing three Acinetobacter-specific envelope proteins.
  • The absence of TssJ is compensated by the TsmK protein, which stabilizes the MC.
  • A novel domain in TssM facilitates homo-oligomerization, essential for T6SS channel formation.

Conclusions:

  • A. baumannii has evolved a unique T6SS MC assembly mechanism.
  • Species-specific proteins (TsmK and TssM) play crucial roles in MC biogenesis and TssJ functional replacement.
  • These findings offer insights into the evolutionary diversity and assembly of bacterial T6SSs.