Related Experiment Video
Updated: Jul 15, 2025
06:30
Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
Published on: August 27, 2021
3.1K
Phages as delivery vehicles and phage display.
Salsabil Makky1, Fatma Abdelrahman1, Maheswaran Easwaran2
1Center for Microbiology and Phage Therapy, Zewail City of Science and Technology, October Gardens, 6th of October City, Giza, Egypt.
Progress in Molecular Biology and Translational Science
|September 28, 2023
Summary
Bacteriophages, or phages, are natural bacterial enemies offering alternatives to antibiotics for combating resistance. Phage display systems are versatile molecular tools with applications in vaccines and treatments for bacterial infections and cancer.
Area of Science:
- Microbiology
- Biotechnology
- Molecular Biology
Background:
- Bacteriophages (phages) are viruses that infect bacteria, recognized as natural predators.
- Antibiotic resistance is a growing global health challenge, necessitating alternative therapeutic strategies.
- Phages possess unique properties, including host specificity and potential as delivery vehicles.
Purpose of the Study:
- To introduce different phage display systems.
- To explore the diverse applications of phage display technology.
- To highlight the role of phages in developing novel vaccines and treatments.
Main Methods:
- Review of existing literature on bacteriophage biology and applications.
- Description of various phage display system constructions and functionalities.
- Analysis of therapeutic and diagnostic approaches utilizing phage display.
Main Results:
- Phage display systems offer a robust platform for molecular and therapeutic applications.
- Phages can be engineered as effective delivery vehicles and display systems.
- Phage display has demonstrated potential in developing vaccines, antibacterial agents, and anti-cancer therapies.
Conclusions:
- Phage display technology is a powerful tool with broad applicability in medicine and biotechnology.
- Phages represent a promising avenue for addressing antibiotic resistance and developing innovative treatments.
- Further research into phage display systems can unlock new therapeutic and diagnostic possibilities.
Related Concept Videos
01:26DNA Bacteriophages
39
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
39
01:30Lytic Cycle of Bacteriophages
70.8K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
70.8K
00:43Lysogenic Cycle of Bacteriophages
62.3K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
62.3K
01:36Delivery Pathways to the Lysosome
6.6K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.6K
01:20Viral Replication: Lytic Cycle
45
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
45
01:25Immune Surveillance by NK Cells and Phagocytes
1.5K
Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
1.5K