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Related Concept Videos

DNA Bacteriophages01:26

DNA Bacteriophages

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Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
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Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

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Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
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Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

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Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
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Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

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In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
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Viral Replication: Lytic Cycle01:20

Viral Replication: Lytic Cycle

45
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
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Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

6.6K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
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Related Experiment Video

Updated: Jul 15, 2025

Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System
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Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System

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Phage for drug delivery vehicles.

Mohit Kumar1, Piyush Parkhey2, Santosh Kumar Mishra3

  • 1Department of Biotechnology, National Institute of Technology, Raipur, Chhattisgarh, India.

Progress in Molecular Biology and Translational Science
|September 28, 2023
PubMed
Summary

Bacteriophages offer a safer alternative to eukaryotic viruses for gene delivery, minimizing risks like immune responses and cancer. Novel phage technologies enable targeted drug delivery, advancing bioinorganic carrier development.

Keywords:
BacteriophageDrug deliveryDrug vehicleFilamentous phagesPathogenicity

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Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
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Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
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Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
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Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Virology

Background:

  • Eukaryotic viruses (adenoviruses, retroviruses, lentiviruses) are common gene delivery vectors but pose risks such as immunogenicity and oncogenesis.
  • The limitations of eukaryotic viruses necessitate the exploration of safer and equally effective alternatives for gene and drug delivery applications.

Purpose of the Study:

  • To explore bacteriophages as a safer and efficient alternative to eukaryotic viruses for drug delivery.
  • To highlight advancements in phage-based technologies for targeted drug delivery and the development of bioinorganic carriers.

Main Methods:

  • Review of current literature on bacteriophage applications in drug delivery.
  • Discussion of novel phage-dependent technologies, including peptide libraries for ligand targeting.
  • Exploration of hybridizing phages with inorganic complexes for bioinorganic carrier construction.

Main Results:

  • Bacteriophages are identified as efficient, reliable, and safer drug delivery vehicles compared to eukaryotic viruses.
  • Phage-based peptide libraries facilitate the recognition and targeting of specific ligands.
  • Hybridization strategies offer potential for creating advanced bioinorganic drug delivery carriers.

Conclusions:

  • Bacteriophages represent a promising platform for developing next-generation drug delivery systems.
  • Advancements in phage technology are paving the way for targeted and safer therapeutic applications.
  • The integration of phages with inorganic materials holds significant potential for novel bioinorganic drug carriers.