Related Experiment Video

Updated: Jul 15, 2025

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

1.6K

SQLE Knockdown inhibits bladder cancer progression by regulating the PTEN/AKT/GSK3β signaling pathway through P53

Fan Zou1, Wu Chen1, Tianbao Song1

  • 1Department of Urology, Renmin Hospital of Wuhan University, 99 ziyang road, Wuhan, 430060, Hubei Province, China.

Cancer Cell International
|September 28, 2023
PubMed

Insights

Squalene epoxidase (SQLE) is upregulated in bladder cancer (BCa), correlating with poor prognosis. Inhibiting SQLE suppresses BCa growth and metastasis via the P53/PTEN/AKT/GSK3β pathway, offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Bladder cancer (BCa) is a prevalent malignancy with limited targeted therapies.
  • Squalene epoxidase (SQLE), a key enzyme in cholesterol biosynthesis, is implicated in cancer progression.
  • Identifying novel therapeutic targets for BCa is crucial for improving patient outcomes.

Purpose of the Study:

  • To investigate the role of SQLE in bladder cancer development and progression.
  • To elucidate the molecular mechanisms underlying SQLE's function in BCa.
  • To evaluate SQLE as a potential therapeutic target for BCa.

Main Methods:

  • Bioinformatic analysis of The Cancer Genome Atlas, The Genotype-Tissue Expression, and Gene Expression Omnibus databases.
  • Quantitative reverse transcription PCR (qRT-PCR) and immunohistochemical staining.
  • In vitro cell culture experiments (SQLE knockdown, rescue experiments) and in vivo tumorigenesis assays in nude mice.

Main Results:

  • SQLE expression is significantly elevated in BCa tissues compared to normal tissues, associated with poor prognosis.
  • SQLE knockdown inhibits BCa cell proliferation and metastasis.
  • SQLE regulates the PTEN/AKT/GSK3β signaling pathway, with P53 identified as a key mediator.

Conclusions:

  • SQLE promotes bladder cancer growth and metastasis through the P53/PTEN/AKT/GSK3β signaling axis.
  • Targeting SQLE presents a promising therapeutic strategy for bladder cancer.
  • This study identifies a novel molecular pathway critical for BCa pathogenesis.
Keywords:
ApoptosisBladder cancerCell cycleP53PTEN/AKT/GSK3β signaling pathwayProliferationSQLE

More Related Videos

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma13:18

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Published on: March 3, 2023

1.3K
Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway07:15

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Published on: August 23, 2024

464

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.8K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
9.0K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K