Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of stocking density on the pharmacokinetics of enrofloxacin and its metabolite ciprofloxacin in rice field eels (Monopterus albus).

Fish physiology and biochemistry·2026
Same author

Multi-source apportionment and speciation drivers of heavy metals in the desert-oasis ecotone.

Journal of environmental sciences (China)·2026
Same author

Foundation model for screening severe mitral regurgitation and severe aortic stenosis from coronary angiograms.

Visual computing for industry, biomedicine, and art·2026
Same author

Trends in mortality and leading causes of death among US women during the menopausal transition, 1999-2023.

The American journal of the medical sciences·2026
Same author

Distribution of Aquatic Vertebrates in the Winter Dry Season Informing Water Resource Management in a Large Floodplain Lake.

Biology·2026
Same author

Mitochondria power the cell's recycling center.

Autophagy·2026

Related Experiment Video

Updated: Jul 15, 2025

Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

46.3K

Quantifying cell viability through organelle ratiometric probing.

Rui Chen1, Kangqiang Qiu2, Guanqun Han1

  • 1Department of Chemistry, University of Cincinnati Cincinnati OH 45221 USA yujie.sun@uc.edu.

Chemical Science
|September 29, 2023
PubMed
Summary

A new fluorescent probe, PCV-1, visualizes cell viability at the single-cell level using super-resolution microscopy. This method enables precise drug toxicity testing and deeper insights into cell death mechanisms.

More Related Videos

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
12:22

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Published on: July 22, 2013

21.1K
Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism Microcystis aeruginosa Using Flow Cytometry
10:16

Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism Microcystis aeruginosa Using Flow Cytometry

Published on: January 29, 2016

9.0K

Related Experiment Videos

Last Updated: Jul 15, 2025

Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

46.3K
Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
12:22

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Published on: July 22, 2013

21.1K
Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism Microcystis aeruginosa Using Flow Cytometry
10:16

Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism Microcystis aeruginosa Using Flow Cytometry

Published on: January 29, 2016

9.0K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Microscopy

Background:

  • Assessing cell viability is critical for cancer research, treatment evaluation, and drug toxicity testing.
  • Conventional methods provide cumulative data from many cells, lacking single-cell resolution.
  • There is a need for advanced techniques to analyze cell viability at the individual cell level.

Purpose of the Study:

  • To develop a novel fluorescent probe (PCV-1) for visualizing cell viability.
  • To enable single-cell level analysis of cell death and injury using super-resolution microscopy.
  • To establish a quantitative assay for assessing individual cell viability.

Main Methods:

  • Design and synthesis of the fluorescent probe PCV-1.
  • Utilization of structured illumination microscopy for super-resolution imaging.
  • Development of the organelle ratiometric probing (ORP) assay by comparing fluorescence in mitochondria and nucleoli.

Main Results:

  • PCV-1 stains mitochondria in live cells and nucleoli in dead cells, reflecting mitochondrial membrane potential and DNA.
  • Dynamic visualization of PCV-1 migration from mitochondria to nucleoli during drug-induced cell injury.
  • The ORP assay with PCV-1 successfully quantified cell viability at the single-cell level, identifying a cytotoxicity cutoff of 0.3.

Conclusions:

  • PCV-1 is the first probe for visualizing cell death and injury under super-resolution imaging.
  • The ORP assay provides a powerful tool for quantitative single-cell viability analysis.
  • This approach offers deeper insights into cell death mechanisms and enhances drug toxicity assessment.