Related Experiment Video
Updated: Jul 15, 2025
Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations
Published on: March 19, 2021
Automated detection of apoptotic bodies and cells in label-free time-lapse high-throughput video microscopy using
Kwan-Ling Wu1, Melisa Martinez-Paniagua1, Kate Reichel1
1William A. Brookshire Department of Chemical and Biomolecular Engineering, University of Houston, Houston, TX 77204, United States.
Motivation:
Reliable label-free methods are needed for detecting and profiling apoptotic events in time-lapse cell-cell interaction assays. Prior studies relied on fluorescent markers of apoptosis, e.g. Annexin-V, that provide an inconsistent and late indication of apoptotic onset for human melanoma cells. Our motivation is to improve the detection of apoptosis by directly detecting apoptotic bodies in a label-free manner.
Results:
Our trained ResNet50 network identified nanowells containing apoptotic bodies with 92% accuracy and predicted the onset of apoptosis with an error of one frame (5 min/frame). Our apoptotic body segmentation yielded an IoU accuracy of 75%, allowing associative identification of apoptotic cells. Our method detected apoptosis events, 70% of which were not detected by Annexin-V staining.
Availability And Implementation:
Open-source code and sample data provided at https://github.com/kwu14victor/ApoBDproject.
