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Liposomal oxygen-generating hydrogel for enhancing cell survival under hypoxia condition
Saeid Moghassemi1, Arezoo Dadashzadeh1, Hafez Jafari2
1Pôle de Recherche en Physiopathologie de la Reproduction, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
Colloids and Surfaces. B, Biointerfaces
|September 29, 2023
Summary
Engineered tissues face oxygen supply challenges. This study developed a liposomal scaffold system that effectively releases oxygen, significantly improving cell viability under hypoxic conditions for tissue engineering applications.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Cell Biology
Background:
- Inadequate oxygen supply to engineered tissues is a major hurdle in tissue engineering and regenerative medicine.
- Hypoxia in engineered tissues can lead to cell death and impaired function.
- Existing methods for oxygenation often face limitations in diffusion and sustained release.
Purpose of the Study:
- To develop and evaluate a novel oxygen-releasing scaffold system for improving cell survival in engineered tissues.
- To address the challenge of oxygen diffusion limitations in tissue implants.
- To provide a cytocompatible solution for cellular oxygenation under hypoxic conditions.
Main Methods:
- Development of a liposomal oxygen-releasing system using catalase-loaded liposomes (CAT@Lip) and hydrogen peroxide-loaded liposomes (H2O2@Lip).
- Incorporation of the liposomal system into an alginate hydrogel scaffold.
- Assessment of cytocompatibility and cell viability of human stromal cells within the scaffold under hypoxic conditions.
- Comparison of cell viability in scaffolds with and without the oxygen-releasing system.
Main Results:
- The oxygen-releasing liposomal scaffold demonstrated high cytocompatibility with human stromal cells.
- Cell viability within the oxygen-releasing liposomal alginate hydrogel under hypoxia was significantly higher (94.62 ± 3.46 %) compared to hydrogel without liposomes (47.18 ± 9.68 %).
- No significant difference in cell viability or apoptosis rate was observed compared to normoxia conditions after three days, confirming the system's effectiveness.
Conclusions:
- The liposomal oxygen-releasing scaffold effectively overcomes oxygen diffusion challenges in tissue implant fabrication.
- This system offers a simple yet potent solution for cellular oxygenation, crucial for advancing tissue engineering.
- The developed technology holds significant potential for improving the success of tissue implants and regenerative medicine therapies.
