BrKCS6 mutation conferred a bright glossy phenotype to Chinese cabbage

Area of Science:

• Plant genetics
• Molecular biology
• Agricultural science

Background:

• Glossy green appearance in leafy vegetables is a desirable commodity trait.
• This trait is primarily due to the absence of epicuticular wax crystals.
• Understanding the genetic basis of this trait is crucial for crop improvement.

Purpose of the Study:

• To identify and clone the gene responsible for the glossy green appearance in Chinese cabbage.
• To characterize the molecular mechanism underlying the glossy trait.
• To explore the potential of the identified gene in breeding programs.

Main Methods:

• Mutagenesis using Ethyl methanesulfonate (EMS).
• Mutant screening and genetic analysis.
• Gene cloning using MutMap and Kompetitive Allele Specific PCR (KASP).
• Molecular characterization including SNP analysis and qRT-PCR.

Main Results:

• Two glossy mutants, wdm9 and wdm10, were identified and controlled by a recessive nuclear gene.
• BrKCS6, encoding 3-ketoacyl-CoA synthases, was identified as the causal gene.
• Specific SNPs in BrKCS6 altered protein structure and significantly reduced gene expression.
• Downstream genes related to BrKCS6 function were also downregulated in mutants.

Conclusions:

• The glossy green trait in Chinese cabbage is attributed to mutations in the BrKCS6 gene.
• BrKCS6 plays a critical role in epicuticular wax formation.
• Cloning BrKCS6 provides a valuable genetic resource for enhancing commodity quality in Chinese cabbage breeding.