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Related Concept Videos

The Blood-brain Barrier00:49

The Blood-brain Barrier

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An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers
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Determination of Blood-Brain Barrier Hyperpermeability Using Intravital Microscopy.

O'lisa Yaa Waithe1, Chinchusha Anasooya Shaji2, Ed W Childs1

  • 1Department of Surgery, Morehouse School of Medicine, Atlanta, GA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 30, 2023
PubMed
Summary
This summary is machine-generated.

This study details intravital microscopy methods to measure blood-brain barrier (BBB) permeability changes in mice after traumatic brain injury (TBI). These findings help understand BBB dysfunction in TBI and related neurological conditions.

Keywords:
Blood-brain barrierIntravital microscopyTraumatic brain injury

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Area of Science:

  • Neuroscience
  • Physiology
  • Biomedical Engineering

Background:

  • The blood-brain barrier (BBB) regulates substance transport into the central nervous system (CNS) via tight junctions.
  • BBB dysfunction, characterized by hyperpermeability, is linked to traumatic brain injury (TBI) and increased intracranial pressure.
  • Intravital microscopy is a key technique for in vivo assessment of BBB permeability.

Purpose of the Study:

  • To describe surgical and imaging methods for assessing BBB permeability changes in the pial microvasculature.
  • To utilize intravital microscopy in a mouse model of TBI to quantify BBB hyperpermeability.

Main Methods:

  • Surgical preparation of the mouse cranial window for pial microvasculature access.
  • In vivo imaging using intravital microscopy to observe and quantify BBB permeability.
  • Induction of TBI in a mouse model to study its effects on BBB integrity.

Main Results:

  • Demonstrated changes in BBB permeability at the pial microvasculature level following TBI.
  • Quantified BBB hyperpermeability using intravital microscopy in a TBI mouse model.
  • Established a reliable method for assessing BBB dysfunction in vivo.

Conclusions:

  • Intravital microscopy provides a robust method for evaluating BBB permeability in TBI models.
  • Understanding BBB changes post-TBI is crucial for developing therapeutic strategies.
  • The described methods facilitate further research into CNS disorders involving BBB disruption.