Quantification of sedative-hypnotics in human urine and plasma via polystyrene-based solid phase extraction-LC-MS/MS...

Area of Science:

• Analytical Chemistry
• Forensic Toxicology
• Clinical Chemistry

Background:

• Overuse of sedative-hypnotics poses health risks, necessitating efficient detection methods.
• Conventional analytical techniques for drug detection have limitations like low throughput and high costs.

Purpose of the Study:

• To develop a novel, high-throughput analytical method for detecting sedative-hypnotics in biological samples.
• To evaluate the precision and reliability of the developed method for clinical and forensic applications.

Main Methods:

• Development of a polystyrene-based solid phase extraction (SPE) technique.
• Utilized liquid chromatography with tandem mass spectrometry (LC-MS/MS) for drug analysis.
• Analyzed twelve common sedative-hypnotics in human urine and plasma samples.

Main Results:

• Achieved good linearity (r ≥ 0.99) in urine (0.1-20 ng/mL) and plasma (0.5-100 ng/mL) for the analytes.
• Demonstrated favorable intra- and inter-day precision, stability, and low limit of quantitation (LLOQ).
• Successfully detected sedative-hypnotics in clinical samples from Hebei General Hospital.

Conclusions:

• The developed SPE-LC-MS/MS method offers a high-throughput and reliable approach for sedative-hypnotic detection.
• The method is effective for toxicology screening, drug abuse monitoring, and clinical diagnosis.
• This analytical tool supports clinical and forensic toxicology laboratories in identifying sedative-hypnotic drug use.