Functionalized DNA nanoplatform for multi-target simultaneous imaging: Establish the atlas of cancer cell species
Xuan Zhao1, Na Na1, Jin Ouyang2
1College of Chemistry, Beijing Normal University, Beijing, 100875, China.
Abstract:
Detection and imaging of cell membrane receptor proteins have gained widespread interest in recent years. However, recognition based on a single biomarker can induce false positive feedback, including off-target phenomenon caused by the absence of tumor-specific antigens. In addition, nucleic acid probes often cause nonspecific and undesired cell internalization during cell imaging. In this work, we constructed a logic gate DNA nano-platform (LGDP) for single-molecule imaging of cell membrane proteins to synergistically diagnose cancer cells. The traffic light-like color response of LGDP facilitates the precise discrimination among different cell lines. Combined with single molecule technology, the target proteins were qualitatively and quantitatively analyzed synergistically. Logic-gated recognition integrated in aptamer-functionalized molecular machines will prompt fast cells analysis, laying the foundation of cancer early diagnosis and treatment.
Insights
This study introduces a novel logic gate DNA nano-platform (LGDP) for precise, single-molecule imaging of cell membrane proteins. This technology enables synergistic cancer cell diagnosis, improving early detection and treatment strategies.
Area of Science:
- Biotechnology
- Molecular Imaging
- Cancer Diagnostics
Background:
- Accurate detection of cell membrane proteins is crucial for cancer diagnosis.
- Single biomarker recognition can lead to false positives and off-target effects.
- Existing nucleic acid probes may cause non-specific cell internalization.
Purpose of the Study:
- To develop a logic gate DNA nano-platform (LGDP) for synergistic diagnosis of cancer cells.
- To enable single-molecule imaging and precise discrimination of different cell lines.
- To improve the accuracy and efficiency of cancer cell analysis.
Main Methods:
- Construction of a logic gate DNA nano-platform (LGDP).
- Utilizing aptamer-functionalized molecular machines for logic-gated recognition.
- Integration with single-molecule technology for qualitative and quantitative analysis.
Main Results:
- LGDP demonstrated a traffic light-like color response for precise cell line discrimination.
- Synergistic qualitative and quantitative analysis of target proteins was achieved.
- The platform facilitated accurate identification of cancer cells.
Conclusions:
- The developed LGDP offers a novel approach for single-molecule imaging of cell membrane proteins.
- Logic-gated recognition enhances the accuracy and speed of cancer cell analysis.
- This technology lays the foundation for early cancer diagnosis and treatment.
