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Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System MMC

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Published on: February 18, 2022

Recent advances in systems metabolic engineering.

Taehee Han¹, Alisher Nazarbekov², Xuan Zou¹

¹Metabolic and Biomolecular Engineering National Research Laboratory and Systems Metabolic Engineering and Systems Healthcare Cross-Generation Collaborative Laboratory, Department of Chemical and Biomolecular Engineering (BK21 four), Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, the Republic of Korea; KAIST Institute for the BioCentury and KAIST Institute for Artificial Intelligence, KAIST, Daejeon 34141, the Republic of Korea; BioProcess Engineering Research Center and BioInformatics Research Center, KAIST, 34141 Daejeon, the Republic of Korea.

Current Opinion in Biotechnology

|October 1, 2023

Summary

This summary is machine-generated.

Systems metabolic engineering creates efficient microbial cell factories for sustainable fuel and material production. This review covers tools and strategies for host cell engineering, from enzymes to genomes, and discusses scaling up production.

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Area of Science:

Biotechnology
Metabolic Engineering
Synthetic Biology

Background:

Systems metabolic engineering integrates multiple disciplines for sustainable bioproduction.
Advancements enable the creation of highly productive microbial cell factories.
This field is crucial for renewable fuels and materials.

Purpose of the Study:

To review recent tools and strategies in systems metabolic engineering.
To highlight emerging trends and integrated approaches.
To discuss the transition from academic research to commercial production.

Main Methods:

Review of enzyme-, genetic module-, pathway-, flux-, genome-, and cell-level engineering tools.
Analysis of integrated strategies combining multiple engineering approaches.
Examination of case studies and examples.

Main Results:

Identification of diverse and advanced engineering tools.
Demonstration of successful applications in microbial cell factory development.
Highlighting of synergistic effects from integrated strategies.

Conclusions:

Systems metabolic engineering is a powerful approach for sustainable bioproduction.
Continued innovation in engineering tools and integrated strategies is key.
Addressing scale-up challenges is vital for commercial viability.