Related Experiment Video
Updated: Jul 15, 2025
Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
Published on: July 27, 2013
Simple Coumarin-Based Fluorescent Probe for Recognition of Pd(II) and Its Live Cell Imaging
Xiangyang Zhang1,2, Yaodan Xu1,2, Youming Shen1,2
1College of Chemistry and Material Engineering, Hunan University of Arts and Science, Changde 415000, P. R. China.
Abstract:
A simple coumarin hydrazine Schiff base bearing a thioether recognition fragment (compound CBBS) has been rationally designed and easily prepared. CBBS exhibited an excellent selectivity for Pd(II) and a low detection limit of 65 nM (S/N = 3). The fluorescence emission intensities of CBBS at 495 nm were linear to Pd(II) concentrations in a wide range from 0 to 80 μM. Moreover, CBBS has been well used in fluorescence imaging of Pd(II) in living A549 cells. CBBS as a simple coordination-based fluorescent probe will inspire the researchers to develop a polymer for selective detection and adsorption of Pd(II).
More Related Videos
Related Concept Videos
03:31Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
01:19Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...