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Updated: Jul 15, 2025
10:46
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
Published on: June 2, 2018
9.4K
Genomic context sensitizes regulatory elements to genetic disruption
Raquel Ordoñez1,2, Weimin Zhang1,2, Gwen Ellis1,3
1Institute for Systems Genetics, NYU School of Medicine, New York, NY 10016, USA.
Biorxiv : the Preprint Server for Biology
|October 2, 2023
Summary
Investigating enhancer function requires understanding genomic context. This study used Big-IN technology to analyze the Igf2/H19 locus, revealing context-dependent enhancer activity and previously unknown regulatory elements.
Area of Science:
- Genomics
- Molecular Biology
- Epigenetics
Background:
- Enhancer function is often studied in isolation, limiting understanding of its role within native genomic contexts.
- The murine Igf2/H19 locus is a model system for studying enhancer selectivity and imprinting control.
Conclusions:
- Genomic context significantly impacts enhancer function, even for well-studied elements.
- The Big-IN approach enables large-scale manipulation of complete loci to study locus architecture and function.
- Findings suggest broader implications for understanding enhancer clusters across the genome.
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