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Updated: Jul 15, 2025
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Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
Published on: April 2, 2016
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Higher-order combinatorial chromatin perturbations by engineered CRISPR-Cas12a for functional genomics
Biorxiv : the Preprint Server for Biology
|October 2, 2023
Summary
Researchers developed multiAsCas12a, a new CRISPR interference tool, enabling higher-order combinatorial genetic targeting. This innovation advances functional genomics by allowing more complex perturbations for biological discovery.
Area of Science:
- Genetics
- Molecular Biology
- Biotechnology
Background:
- Multiplexed genetic perturbations are essential for understanding gene interactions.
- CRISPR interference (CRISPRi) offers a non-genotoxic method for perturbing genetic elements, especially non-coding regions.
- Current CRISPRi pooled screening is limited to perturbing only 1-3 genomic sites per cell.
Conclusions:
- The engineered multiAsCas12a variant enables efficient, higher-order combinatorial CRISPRi screening.
- This tool provides a powerful group testing framework for biological discovery and engineering.
- Facilitates the efficient surveying of numerous chromatin perturbation combinations.
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