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Improvement of the mismatch amplification mutation assay-PCR for discrimination between Streptococcus suis serotypes
Erina Okuhama-Yoshida1, Mizuki Nakayama1, Miki Hattori2
1Miyazaki Prefectural Livestock Hygiene Service Center, 3151-1 Shimonaka, Sadoharacho, Miyazaki 880-0212, Japan.
Journal of Microbiological Methods
|October 2, 2023
Abstract:
A mismatch amplification mutation assay (MAMA)-PCR, which detects a single-nucleotide polymorphism contributed to serological difference between Streptococcus suis serotypes 2 and 1/2, is used to discriminate between these serotypes. The present study reports unusual serotype 1/2 isolates untypable by the MAMA-PCR and improvement of the MAMA-PCR for typing such isolates.
