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Liver endothelium desialates ceruloplasmin
Biochemical and Biophysical Research Communications
|October 15, 1986
Summary
Liver endothelium desialates ceruloplasmin (CP) during transport. Hepatocytes then internalize this desialated CP via asialoglycoprotein receptors, clarifying CP
Area of Science:
- Biochemistry
- Cell Biology
- Hepatology
Background:
- Ceruloplasmin (CP) transport across liver endothelium is receptor-mediated.
- CP is externalized on the abluminal side after endothelial transport.
Purpose of the Study:
- To investigate the modification of CP during liver endothelial transport.
- To elucidate the mechanism of CP uptake by hepatocytes.
Main Methods:
- Utilized 125I or 3H-labeled ceruloplasmin for transport studies.
- Employed affinity chromatography with an RCA120 column to analyze the supernate.
- Investigated CP modification during passage through liver endothelium.
Main Results:
- Liver endothelium completely desialates ceruloplasmin during its transport.
- Desialated CP is efficiently removed by hepatocytes.
- Asialoglycoprotein receptors on hepatocytes mediate the uptake of desialated CP.
Conclusions:
- The liver endothelium modifies ceruloplasmin by desialation.
- Hepatocyte asialoglycoprotein receptors play a crucial role in clearing desialated ceruloplasmin.