Related Experiment Videos
[A simplified variant of the Maxam-Gilbert method for determining the primary structure of oligonucleotides and DNA
Bioorganicheskaia Khimiia
|September 1, 1986
Abstract:
A modification to the Maxam-Gilbert method is proposed that involves precipitation of the nucleotide material with the acetone solution of lithium perchlorate after the completion of chemical reactions to remove the reagents. Modification of cytosine residues is carried out in the presence of lithium chloride. The new mode of precipitation simplifies and speeds up the analysis of oligonucleotides and DNA fragments.