Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A microcomputer system for video image analysis and diagnostic microdensitometry.

L R Jarvis

    Analytical and Quantitative Cytology and Histology
    |September 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The development of the cuticle in Phormium tenax.

    Planta·2014
    Same author

    The microcomputer and image analysis in diagnostic pathology.

    Microscopy research and technique·1992
    Same author

    Video image analysis of AgNOR distribution in the normal and adenomatous colorectum.

    The Journal of pathology·1992
    Same author

    Two methods of assessment of methotrexate hepatotoxicity in patients with rheumatoid arthritis.

    Annals of the rheumatic diseases·1991
    Same author

    The use of computer-assisted video image analysis in the enumeration of immuno-stained cells in tissue sections.

    Journal of immunological methods·1990
    Same author

    S100 protein, neurone specific enolase, and nuclear DNA content in Spitz naevus.

    The Journal of pathology·1990

    A new microdensitometry system uses a microcomputer and solid-state camera for accurate DNA content analysis in cell nuclei. This cost-effective tool offers rapid, repeatable performance for diagnostic applications.

    Area of Science:

    • Biomedical Engineering
    • Cell Biology
    • Image Analysis

    Background:

    • Microdensitometry is crucial for quantitative cell analysis.
    • Existing methods may be time-consuming or require specialized equipment.
    • There is a need for accessible, accurate microdensitometry systems.

    Purpose of the Study:

    • To develop a practical microdensitometry system using a microcomputer and solid-state camera.
    • To enable accurate and efficient DNA content distribution analysis.
    • To provide a cost-effective alternative for diagnostic applications.

    Main Methods:

    • Development of a microdensitometry system integrating a microcomputer, video digitizer, and solid-state camera.
    • Implementation of image analysis and densitometry with user-friendly control for editing and calibration.

    Related Experiment Videos

  • Utilizing the linear photometric properties of the imaging device for high-accuracy measurements.

    • Main Results:

    • The developed system demonstrated rapid and repeatable performance in determining DNA content distribution.
    • Measurements were performed on Feulgen-stained cell nuclei.
    • The system's linear photometric properties ensured high measurement accuracy.

    Conclusions:

    • A practical image analysis microdensitometer can be effectively designed using readily available microcomputer technology.
    • The system offers a low-cost, simple-to-operate solution for diagnostic applications.
    • This microdensitometer is beneficial when flow cytometry is not feasible or automated systems are unavailable.