Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Extracellular Matrix01:42

The Extracellular Matrix

82.7K
Overview
82.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prenatal diagnosis of congenitally calcified tricuspid valve with postnatal follow-up and outcomes.

Annals of pediatric cardiology·2026
Same author

Editorial: Robotic applications for a sustainable future.

Frontiers in robotics and AI·2026
Same author

Ruthenium(II)-catalyzed regioselective C8-H acyloxylation of indolizines with carboxylic acids.

Chemical communications (Cambridge, England)·2026
Same author

Virtual reality in planning complex heart surgery.

The Journal of thoracic and cardiovascular surgery·2026
Same author

Chromatin-derived histone proteins from <i>Schmidtea mediterranea</i> function as innate antimicrobial effectors.

Frontiers in cellular and infection microbiology·2026
Same author

Bioinspired zein-chitosan nanocomplexes for co-delivery of 5-fluorouracil and silibinin: Optimization, characterization and anticancer activity in colorectal cancer.

International journal of biological macromolecules·2026

Related Experiment Video

Updated: Jul 15, 2025

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
08:41

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Published on: December 4, 2017

9.1K

Biocompatible Human Placental Extracellular Matrix Derived Hydrogels.

Sunil Gujjar1, Anurag Tyagi1, Saloni Sainger1

  • 1Biomaterials Laboratory, Translational Health Science and Technology Institute, Faridabad, Haryana, 121001, India.

Advanced Biology
|October 3, 2023
PubMed
Summary

Acellular placental extracellular matrix (P-ECM) hydrogels show promise for soft tissue reconstruction, supporting cell growth. Gamma irradiation hinders hydrogel stability, necessitating alternative sterilization methods.

Keywords:
biocompatibilitydecellularizationhydrogelsplacental tissuesterilization

More Related Videos

Ultrathin Porated Elastic Hydrogels As a Biomimetic Basement Membrane for Dual Cell Culture
11:34

Ultrathin Porated Elastic Hydrogels As a Biomimetic Basement Membrane for Dual Cell Culture

Published on: December 26, 2017

7.8K
Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel
08:23

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel

Published on: October 13, 2018

10.5K

Related Experiment Videos

Last Updated: Jul 15, 2025

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
08:41

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Published on: December 4, 2017

9.1K
Ultrathin Porated Elastic Hydrogels As a Biomimetic Basement Membrane for Dual Cell Culture
11:34

Ultrathin Porated Elastic Hydrogels As a Biomimetic Basement Membrane for Dual Cell Culture

Published on: December 26, 2017

7.8K
Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel
08:23

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel

Published on: October 13, 2018

10.5K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Extracellular matrix (ECM) hydrogels offer versatile applications in vitro and in vivo.
  • Human placental tissue is a potential source for developing novel biomaterials.
  • Decellularization techniques are crucial for creating acellular ECM scaffolds.

Purpose of the Study:

  • To prepare and characterize placental extracellular matrix (P-ECM) hydrogels from human placental tissue.
  • To evaluate the physical and biological properties of P-ECM hydrogels for soft tissue reconstruction.
  • To assess the impact of gamma irradiation and HCl treatment on P-ECM hydrogel stability and biocompatibility.

Main Methods:

  • Human placental tissue was decellularized using a perfusion-based approach with detergents and enzymes.
  • Acellular placental tissue powder (P-ECM) was homogenized and used to prepare hydrogels.
  • P-ECM hydrogels were evaluated for mechanical properties, cell proliferation, and viability.
  • The effect of gamma irradiation and HCl treatment on hydrogel formation and sterility was investigated.

Main Results:

  • Decellularization significantly reduced cellular content while preserving major ECM proteins.
  • Increasing P-ECM concentration enhanced hydrogel mechanical properties.
  • P-ECM hydrogels demonstrated good biocompatibility, supporting cell proliferation and viability.
  • Gamma irradiation prevented stable hydrogel formation, whereas HCl treatment reduced bacterial bioburden.

Conclusions:

  • P-ECM hydrogels possess favorable physical and biological characteristics for soft tissue reconstruction.
  • These hydrogels create a conducive microenvironment for cellular growth.
  • Innovative sterilization methods are required for P-ECM hydrogel applications.