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Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells
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Intersection clock reveals a rejuvenation event during human embryogenesis.

Csaba Kerepesi1,2, Vadim N Gladyshev1

  • 1Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.

Aging Cell
|October 3, 2023
PubMed
Summary

Human embryos undergo a rejuvenation event during early development, similar to mice. This finding, observed around gastrulation, suggests a conserved biological process across species.

Keywords:
RRBSWGBSagingbisulfite sequencingepigenetic clockhuman embryogenesisrejuvenation

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Area of Science:

  • Developmental Biology
  • Epigenetics
  • Genomics

Background:

  • A rejuvenation event was previously identified in early mouse development.
  • The existence of a similar event in human embryogenesis remained unconfirmed.

Purpose of the Study:

  • To investigate epigenetic age dynamics during human embryogenesis.
  • To determine if human embryos experience a developmental rejuvenation event.

Main Methods:

  • Development of a novel epigenetic clock method, the "intersection clock," utilizing bisulfite sequencing.
  • Application of the intersection clock to human embryo development and pluripotent stem cell datasets.

Main Results:

  • Epigenetic age showed no significant change from cleavage to blastocyst stages in human embryos.
  • A significant decrease in epigenetic age was observed between blastocysts and epiblast cells.
  • Epigenetic age decreased in postimplantation samples compared to preimplantation samples.
  • Primed pluripotent stem cells exhibited significantly lower epigenetic age than naïve pluripotent stem cells.

Conclusions:

  • Human embryos experience a rejuvenation event during early embryogenesis, conserved with mice.
  • This rejuvenation event occurs around the gastrulation stage in both species.
  • The "intersection clock" offers a new tool for epigenetic age studies using bisulfite sequencing data.