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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jul 15, 2025

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

OMIP-097: High-parameter phenotyping of human platelets by spectral flow cytometry.

Benjamin E J Spurgeon¹, Andrew L Frelinger¹

¹Center for Platelet Research Studies, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Harvard Medical School, Boston, Massachusetts, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|October 3, 2023

Summary

This study presents a 16-color spectral flow cytometry panel for detailed analysis of platelet function and phenotype in whole blood. The optimized panel enables high-parameter phenotyping of procoagulant platelets and distinguishes classic from nonclassic platelet functions.

Keywords:

biomarkers blood platelets flow cytometry phenotype platelet activation

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Last Updated: Jul 15, 2025

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

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Published on: November 22, 2024

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

Area of Science:

Immunology
Hematology
Biotechnology

Background:

Platelets play crucial roles in hemostasis, inflammation, and immune responses.
Characterizing platelet phenotype and function is essential for understanding various diseases.
Current methods may lack the resolution to comprehensively analyze diverse platelet populations.

Purpose of the Study:

To develop and validate a comprehensive 16-color spectral flow cytometry panel for high-parameter analysis of human platelet phenotype and function.
To enable detailed phenotyping of procoagulant platelets and distinguish between classic and nonclassic platelet functions.

Main Methods:

Development of a 16-color spectral flow cytometry panel for whole blood analysis.
Inclusion of established and novel markers for comprehensive platelet phenotyping.
Optimization of protocols for high-parameter analysis of procoagulant platelets.
Demonstration of automatic gating for efficient platelet analysis.

Main Results:

The panel successfully analyzes platelet phenotype and function, including procoagulant platelets.
Established markers (e.g., CD62P, PAC-1) allow subsetting of classic platelet phenotypes.
Novel markers (e.g., TLR9) enable resolution of platelets with nonclassic functions.
Multiple inducible and constitutive markers are measurable, facilitating broad platelet characterization.

Conclusions:

The developed 16-color panel provides a powerful tool for high-parameter platelet analysis in research and clinical settings.
This panel facilitates the comprehensive study of platelet heterogeneity and function.
The panel is adaptable for specific research needs, offering broad applicability.