Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

13.1K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
13.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Iatrogenic constipation in gastrointestinal surgery.

Journal of visceral surgery·2022
Same author

Remnant cystic duct cholangiocarcinoma mimicking hepatic pedicle lymph node.

Diagnostic and interventional imaging·2020
Same author

Intestinal microbiota contributes to individual susceptibility to alcoholic liver disease.

Gut·2015
Same author

Subversion of human intestinal mucosa innate immunity by a Crohn's disease-associated E. coli.

Mucosal immunology·2014
Same author

[Nonalcoholic fatty liver disease in a severely obese adolescent. An arguable liver biopsy].

Archives de pediatrie : organe officiel de la Societe francaise de pediatrie·2010
Same author

The Lactobacillus plantarum strain ACA-DC287 isolated from a Greek cheese demonstrates antagonistic activity in vitro and in vivo against Salmonella enterica serovar Typhimurium.

Journal of applied microbiology·2007

Related Experiment Video

Updated: Jul 15, 2025

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
07:23

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

Published on: September 5, 2013

43.9K

A flow cytometry method for safe detection of bacterial viability.

S Servain-Viel1, M-L Aknin1, S Domenichini2

  • 1Plateforme CYM - UMS-IPSIT, Université Paris-Saclay, Inserm, CNRS, Ingénierie et Plateformes au Service de l'Innovation Thérapeutique, Orsay, France.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|October 3, 2023
PubMed
Summary

Researchers developed a safer flow cytometry protocol for bacterial viability assessment. This method uses non-carcinogenic dyes and a pre-fixation viability labeling step, reducing risks to staff and equipment.

Keywords:
bacteriaflow cytometry - viability dyehealth and safetyprevention

More Related Videos

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
08:23

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Published on: March 2, 2020

12.8K
Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
06:39

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

Published on: June 30, 2023

1.1K

Related Experiment Videos

Last Updated: Jul 15, 2025

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
07:23

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

Published on: September 5, 2013

43.9K
Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
08:23

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Published on: March 2, 2020

12.8K
Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
06:39

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

Published on: June 30, 2023

1.1K

Area of Science:

  • Microbiology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Flow cytometry is crucial for bacterial population analysis in research.
  • Existing protocols pose risks due to hazardous reagents and potential exposure.
  • There is a need for safer, reliable methods for bacterial viability assessment.

Purpose of the Study:

  • To develop a safe flow cytometry protocol for bacterial viability measurement.
  • To minimize risks of biological contamination and chemical exposure for staff and equipment.
  • To identify non-carcinogenic viability dyes suitable for bacterial analysis.

Main Methods:

  • A novel protocol involving pre-fixation viability labeling using eFluor Fixable Viability dyes.
  • Incorporation of bacterial DNA labeling (SYTO or DRAQ5) to distinguish bacteria from background signals.
  • Validation of three viability-DNA marker combinations on LSR Fortessa with various bacterial cultures.

Main Results:

  • The developed protocol successfully measured bacterial viability in pure and mixed cultures.
  • The method demonstrated reliability across different bacterial types and cytometer platforms.
  • Results were validated using confocal microscopy and time-course culture monitoring.

Conclusions:

  • The new protocol offers a safer alternative for bacterial viability assessment using flow cytometry.
  • It reduces reliance on hazardous reagents like propidium iodide.
  • The protocol is adaptable and opens avenues for further bacterial characterization.