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Low-input and single-cell methods for Infinium DNA methylation BeadChips.

Sol Moe Lee, Christian E Loo, Rexxi D Prasasya

    Biorxiv : the Preprint Server for Biology
    |October 3, 2023
    PubMed
    Summary

    Researchers optimized DNA methylation profiling for small cell samples. New methods significantly improve detection rates from single cells, enabling detailed epigenome analysis in limited DNA samples.

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    Area of Science:

    • Epigenetics
    • Genomics
    • Molecular Biology

    Background:

    • The Infinium BeadChip is a standard for DNA methylome profiling but requires substantial DNA input (>200 ng).
    • This high input requirement limits its use in low cell-number samples, such as primordial germ cells.
    • There is a need for optimized workflows to enable DNA methylation analysis on limited DNA quantities.

    Conclusions:

    • Comprehensive experimental and computational solutions were developed to enable DNA methylome profiling with limited DNA input.
    • The optimized workflow significantly extends the utility of the Infinium BeadChip technology for population-scale epigenome studies involving scarce biological samples.
    • This advancement facilitates detailed epigenomic investigations in cell types previously inaccessible due to DNA quantity limitations.