Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A novel homozygous C15ORF41 variant (c.251T > A; p.Val84Glu) causing congenital dyserythropoietic anemia type Ib in an Indian patient: molecular and structural evidence.

Molecular biology reports·2026
Same author

Henoch-Schoenlein purpura (HSP) like lesions in IL12RB1 and IL12B defects - A multi-centric experience from India.

Clinical and experimental immunology·2026
Same author

Underutilisation of hydroxyurea in sickle cell disease: a global scoping review of multilevel barriers and facilitators.

Journal of community genetics·2026
Same author

Spectrum of autoantibodies and other serological parameters in connective tissue disease-associated interstitial lung disease patients.

Frontiers in medicine·2026
Same author

Mendelian susceptibility to mycobacterial disease: Clinical, immunological, and genetic study in India.

Journal of human immunity·2026
Same author

Novel <i>ABCG5</i> and <i>ABCG8</i> Variants in Sitosterolemia: Insights Into Haemolysis, Calcium Dysregulation and Therapeutic Challenges.

Human mutation·2026

Related Experiment Video

Updated: Jul 15, 2025

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
08:44

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Published on: September 7, 2022

2.5K

Normative data for paediatric lymphocyte subsets: A pilot study from western India.

Neha Jodhawat1, Umair Ahmed Bargir2, Priyanka Setia2

  • 1Department of Pediatric Immunology & Leukocyte Biology, Indian Council of Medical Research - National Institute of Immunohaematology, KEM Hospital, Mumbai, aharashtra, India.

The Indian Journal of Medical Research
|October 3, 2023
PubMed
Summary

This study establishes reference values for lymphocyte subsets in Indian children, crucial for diagnosing immunodeficiencies. Findings reveal age-related changes in T, B, and natural killer cells, aiding accurate pediatric immunological assessments.

Keywords:
Childrenflow cytometrylymphocyte subsetpilot studyprimary immunodeficiency

More Related Videos

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children
06:22

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

Published on: February 10, 2023

1.0K
Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
07:39

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Published on: January 7, 2019

12.3K

Related Experiment Videos

Last Updated: Jul 15, 2025

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
08:44

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Published on: September 7, 2022

2.5K
Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children
06:22

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

Published on: February 10, 2023

1.0K
Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
07:39

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Published on: January 7, 2019

12.3K

Area of Science:

  • Immunology
  • Pediatric Hematology

Background:

  • Accurate diagnosis of immunodeficiencies requires age-matched reference values.
  • India lacks established reference values for rare lymphocyte subsets, relying on international data.
  • Limited data exists on normal variations of rare lymphocyte subsets in Indian children.

Purpose of the Study:

  • To establish normative values for clinically important lymphocyte subsets in Indian children across different age groups.
  • To address the lack of country-specific reference ranges for pediatric immunophenotyping.

Main Methods:

  • A pilot study enrolled 148 children aged 0-16 years.
  • Flow cytometry was used to determine lymphocyte subsets, including T, B, and natural killer cells.
  • Data was analyzed to obtain median values and percentiles for 34 lymphocyte sub-populations.

Main Results:

  • Age-related trends were observed: decreasing T and B naive cells, increasing memory cells.
  • Activated T cell subsets increased up to one year and then declined.
  • TCRgd+T cell percentage increased with age, while double negative T cells remained stable.

Conclusions:

  • This study provides preliminary data for establishing pediatric reference ranges in India.
  • Future large-scale, multi-centric studies are needed to validate these findings.
  • Accurate reference ranges will aid clinicians in diagnosing various pediatric immune disorders.