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Human osteosarcoma cells in response to ELF-MF: Morphological remodeling compared to cell proliferation
Amin Barati Shoorche1, Alireza Mohammadkarim1, Majid Jadidi1
1Department of Medical Physics, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Journal of Cancer Research and Therapeutics
|October 3, 2023
Summary
Extremely low-frequency electromagnetic fields (ELF-MF) altered human osteosarcoma cells, decreasing proliferation and increasing fractal dimensions after fractionated exposure. Fractal dimension analysis offers a new index for predicting structural changes in these cells.
Area of Science:
- Biophysics
- Cell Biology
- Electromagnetic Field Research
Background:
- Osteosarcoma is a significant bone cancer.
- Understanding cellular responses to electromagnetic fields is crucial for potential therapeutic applications.
Purpose of the Study:
- To investigate the structural changes in human osteosarcoma cells exposed to extremely low-frequency electromagnetic fields (ELF-MF).
- To analyze the relationship between fractal dimension and cell proliferation rate in radiation-induced osteosarcoma cells.
Main Methods:
- Human osteosarcoma MG-63 cells were exposed to 2-mT magnetic fields (sinusoidal, triangular, pulsed) continuously (3h) or fractionated (3 days).
- Cell proliferation was assessed using proliferation assays.
- Cell shape index was determined via immunofluorescence staining and confocal imaging.
- Fractal dimension analysis was performed on morphological images.
Main Results:
- Cell proliferation and shape index significantly decreased in irradiated osteosarcoma cells compared to controls.
- Fractal dimensions showed a significant increase following fractionated ELF-MF exposure over 3 consecutive days.
Conclusions:
- Fractal dimensions serve as a novel morphological index for predicting structural remodeling in human osteosarcoma cells subjected to fractionated ELF-MF irradiation.
- Different waveforms of ELF-MF induced similar effects on cellular morphological remodeling and proliferation.
