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Development and validation of real-time recombinase polymerase amplification-based assays for detecting HPV16 and

Jiaxu Ying1,2,3, Lingjing Mao1,2,3, Yujing Tang4

  • 1Centre for Microbes, Development and Health, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Unit of Discovery and Molecular Characterization of Pathogens , Shanghai, China.

Microbiology Spectrum
|October 3, 2023
PubMed
Abstract

Keywords:
cervical cancerhuman papillomavirusreal-time recombinase polymerase amplification

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...