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Integrated Microwell Array-Based Microfluidic Chip with a Hand-Held Smartphone-Controlled Device for Nucleic Acid

Haiying Shen1, Lianhua Dong1, Yunhua Gao1

  • 1National Institute of Metrology, Beijing 100029, People's Republic of China.

Analytical Chemistry
|October 3, 2023
PubMed
Summary

This study presents an integrated microfluidic chip for rapid nucleic acid detection. The portable, smartphone-controlled device enables easy point-of-care testing anywhere with internet access.

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Molecular Diagnostics

Background:

  • Nucleic acid detection is crucial for disease diagnosis.
  • Current methods can be complex and require specialized equipment.
  • There is a need for portable, user-friendly diagnostic platforms.

Purpose of the Study:

  • To design a highly integrated microfluidic chip for nucleic acid extraction, amplification, and detection.
  • To develop a compact, hand-held device for operating the chip.
  • To enable smartphone-controlled, internet-enabled nucleic acid testing.

Main Methods:

  • Utilized magnetic beads for nucleic acid capture in microwell arrays.
  • Developed on-chip liquid handling via manual syringe operation.
  • Integrated a hand-held device with temperature control and imaging capabilities.
  • Employed reverse transcription loop-mediated isothermal amplification (RT-LAMP).

Main Results:

  • Achieved one-well-one-bead magnetic bead trapping.
  • Demonstrated successful nucleic acid extraction, amplification, and detection of SARS-CoV-2 pseudoviruses.
  • Validated the hand-held device's performance for RT-LAMP.
  • Confirmed smartphone control and data analysis via internet connectivity.

Conclusions:

  • A highly integrated, easy-to-operate microfluidic chip and hand-held device were successfully developed.
  • The platform enables rapid nucleic acid detection.
  • This system shows significant potential for mobile point-of-care testing (POCT).