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Circulating transferrin receptor in human serum.

Y Kohgo, T Nishisato, H Kondo

    British Journal of Haematology
    |October 1, 1986
    PubMed
    Summary
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    Researchers detected circulating transferrin receptor in human serum using a sensitive immunoassay. Elevated levels were observed in patients with hematological malignancies, indicating potential diagnostic value.

    Area of Science:

    • Biochemistry
    • Hematology
    • Immunology

    Background:

    • Transferrin receptor (TfR) is crucial for iron uptake.
    • Serum TfR levels may reflect cellular TfR expression and iron status.
    • Sensitive detection methods are needed to quantify serum TfR.

    Purpose of the Study:

    • To develop and validate a sensitive immunoassay for human serum transferrin receptor.
    • To determine normal serum TfR concentrations in healthy adults.
    • To investigate serum TfR levels in patients with hematological malignancies.

    Main Methods:

    • Sensitive immunoassay for serum transferrin receptor detection.
    • Polyacrylamide gel electrophoresis-immunoblotting for molecular weight analysis.

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    Main Results:

    • Mean serum transferrin receptor concentrations in healthy males and females were 251 +/- 94 ng/ml and 256 +/- 99 ng/ml, respectively.
    • Serum TfR concentrations varied widely in patients with hematological malignancies (acute leukemia, multiple myeloma, malignant lymphoma), ranging from normal to 1100 ng/ml.
    • Electrophoresis-immunoblotting revealed a single band of 80-100 kDa in serum.

    Conclusions:

    • A sensitive immunoassay can reliably detect circulating transferrin receptor in human serum.
    • Serum transferrin receptor levels are elevated in patients with hematological malignancies.
    • Serum transferrin receptor may serve as a biomarker for diagnosing and monitoring hematological cancers.