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Cell Microencapsulation within Gelatin-PEG Microgels Using a Simple Pipet Tip-Based Device.

Thuy P T Nguyen1, Fanyi Li2, Brendan Hung1

  • 1Department Materials Science and Engineering, Monash University, Clayton, Victoria 3800, Australia.

ACS Biomaterials Science & Engineering
|October 3, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a simple pipet-based chip to create tunable, cell-laden microgels for tissue engineering. This method enables controlled microgel production for various tissue regeneration applications.

Keywords:
cell encapsulationhydrogelmicrofluidicmicrogel

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Microfluidics

Background:

  • Microgels, hydrogel microparticles, are promising for tissue engineering due to their ability to support cell interactions and facilitate nutrient exchange.
  • Their high surface area-to-volume ratio is crucial for effective nutrient and metabolite transport in engineered tissues.

Purpose of the Study:

  • To present methods for fabricating and modifying microfluidic devices for microgel production.
  • To demonstrate the encapsulation of mesenchymal stromal cells within these microgels for tissue engineering applications.

Main Methods:

  • Fabrication of microfluidic devices using common lab consumables like pipet tips and tubing.
  • Controlled generation of microgels with sizes ranging from 130-800 μm.
  • Encapsulation of mesenchymal stromal cells via photo-cross-linking of gelatin-norbornene and PEG dithiol.

Main Results:

  • Successful production of microgels in a controlled size range (130-800 μm).
  • Demonstrated efficient encapsulation of mesenchymal stromal cells within the microgels.
  • Developed a versatile and easily assembled pipet-based chip for microgel fabrication.

Conclusions:

  • The pipet-based chip provides a simple, tunable, and versatile method for producing cell-laden microgels.
  • This approach facilitates the creation of macroporous tissue constructs for diverse tissue engineering applications.
  • The technology is readily implementable in most laboratories for advancing tissue regeneration research.