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Both sexes develop DKD in the CD1 uninephrectomized streptozotocin mouse model
Jackie Trink1, Ifeanyi Kennedy Nmecha1, Dan Zhang1
1Division of Nephrology, St. Joseph's Hospital, McMaster University, 50 Charlton Ave East, Rm T3311, Hamilton, ON, L8N 4A6, Canada.
Scientific Reports
|October 3, 2023
Summary
Sex influences diabetic kidney disease (DKD) progression in mice. While both sexes develop DKD characteristics, females show greater albuminuria and kidney hypertrophy, while males exhibit higher blood pressure and glomerular changes.
Area of Science:
- Nephrology
- Endocrinology
- Immunology
Background:
- Diabetic kidney disease (DKD) is a leading cause of kidney failure.
- Sex is increasingly recognized as a critical factor in DKD pathogenesis.
- Existing DKD research predominantly uses male animal models.
Purpose of the Study:
- To investigate the influence of biological sex on the development of diabetic kidney disease in a murine model.
- To compare clinical and pathological outcomes between male and female mice with streptozotocin-induced diabetes.
Main Methods:
- CD1 mice underwent unilateral nephrectomy and streptozotocin injection to induce type 1 diabetes.
- Dosage adjustment of streptozotocin was necessary for female mice due to higher mortality.
- Mice were monitored for 12 weeks to assess hyperglycemia, albuminuria, kidney hypertrophy, blood pressure, and fibrotic markers.
Main Results:
- Both male and female mice developed comparable hyperglycemia.
- Females exhibited greater increases in albuminuria and glomerular volume, and developed kidney hypertrophy.
- Males showed a more significant rise in blood pressure and glomerular basement membrane thickening.
Conclusions:
- Both sexes develop clinical and pathological features of early diabetic kidney disease.
- Observed sex-specific differences in DKD progression highlight the need for sex-inclusive research.
- This model is suitable for evaluating sex-specific therapeutic interventions for DKD.
